Acetylcholinesterase Inhibition in Organophosphate-Resistant Rhyzopertha dominica (F.) (Coleoptera: Bostrichidae)
نویسندگان
چکیده
RESUMO Populações de Rhyzopertha dominica (F.) resistente e susceptível a inseticidas fosforados foram investigadas para verificar a ocorrência de acetilcolinesterase alterada. Malaoxon, a diferentes concentrações, foi utilizado como inibidor em ensaios colorimétricos "in vitro". Populações resistentes provenientes dos municípios de Patrocínio (Minas Gerais, Brasil) e Riley (Kansas, E.U.A.) apresentaram, respectivamente, 9,3e 12,3vezes menor sensibilidade ao malaoxon que a população susceptível de laboratório. Acetilcolinesterase de insetos resistentes foi inibida mais lentamente que a enzima de insetos susceptíveis com razão de constante bimolecular (k i ) diferindo em 5,5e 12,8vezes para o malaoxon. Esses resultados confirmaram estudos prévios indicando o envolvimento de acetilcolinesterase alterada como mecanismo de resistência a inseticidas organofosforados em populações brasileiras e norteamericanas de R. dominica.
منابع مشابه
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تاریخ انتشار 2006